Baculovirus vectors have proven useful in producing high levels of biologically active eukaryotic proteins and providing cellular fractions which are enriched in the protein of interest. Expression occurs in infected insect cells which also provide a suitable environment for posttranslational modification and folding of the protein product. Stable baculovirus vectors can be constructed rapidly with a minimum of viral manipulation.

Alexithymia is a subclinical trait involving difficulty describing and identifying emotions. It is common in a number of psychiatric conditions. Alexithymia in children is sometimes measured by parent report and sometimes by child self-report, but it is not yet known how closely related the two measures are. This is an important question both theoretically and practically, in terms of research design and clinical practice. We conducted a preliminary study to investigate this question in a sample of 6- to 11-year-old neurotypical (...) children and their parents. Parent and child reports were not correlated, and 93% of parents under-estimated their child’s level of alexithymia relative to the child’s self-report. Based on these results, we hypothesize that when asked to report on the child’s alexithymia, children and parents may not be reporting on the same phenomenon, and thus these two measures may not be interchangeable. These provocative findings, however, must be considered preliminary: our analyses were sufficiently powered to detect a strong relation between the two types of report had one existed, but our analyses were not sufficiently powered to distinguish between a small relation and no relation at all. (shrink)

The aim of this paper is to develop an adequate version of the D-N theory of explanation for particular events and to show how the resulting D-N model can be used as a tool in articulating a regularity theory of causation and an analysis of the truth conditions for counterfactual conditionals. Starting with a basic model that is largely the product of other workers in this field, two new restrictions are formulated in order to construct a version of D-N explanation (...) that does not yield the counterintuitive results that have plagued all earlier versions. An additional condition is then developed that is indispensable for utilizing the D-N model as a tool in formulating a regularity theory of causation. Finally, it is shown how a suitable model of potential D-N explanation facilitates the formulation of a theory of counterfactual conditionals. (shrink)

Philosophical Investigations, EarlyView.

In humanities departments across the country Allan Bloom is condemned and reviled—but for what misconduct? The present investigation into our distorted view of Bloom begins by reframing his transgressions. Bloom has offended eminent philosophy professors by boosting students' desire for a philosophic education. He has emboldened promising young people by teaching them to bear up under the deforming forces of convention and corruption and edge their way towards the question, ‘How should I live my life?’ A philosophic education as Bloom (...) sees it ‘means precisely helping students to pose this question to themselves, to become aware that the answer is neither obvious nor simply unavailable, and that there is no serious life in which this question is not a continuous concern’. The philosopher's interests are not shared by most people. Hostility to philosophy is the natural condition of man. This surprising fact, plainly stated, invites so much anger that Bloom—standing in for the philosopher—is forced to defend himself. The story of Bloom, like Socrates', gives an account of philosophy's emergence, distinctive features and always imaginable suppression. Plato's artful writing captured Socrates' way of life for posterity. This essay explores the nature and function of Bloom's bestselling book The Closing of the American Mind (1987), as well as the purpose of Saul Bellow's final novel, Ravelstein (2000), a fictive account of Bloom's life and death. Together these books amount to the true Apology of Allan Bloom. (shrink)

It is widely agreed that ‘scientific law’ is one of the key scientific terms which any adequate philosophy of science must attempt to clarify or define. The importance of the concept ‘law’ is made evident by the fact that the distinctive functions of science—explanation and prediction—are usually analyzed with reference to laws. Thus events are explained by showing that descriptions of them are deducible from laws, and laws are utilized in deducing descriptions of unknown future events, thereby permitting their prediction. (...) Moreover, it has recently become clear that the concept ‘law’ is relevant to the analysis of counterfactual conditionals, disposition terms, and the physical modalities. An adequate explication of ‘law’, then, will further the analysis of these important concepts. (shrink)

The proposal regarding rules and similarity is considered in terms of ability to provide insights regarding previous work on reasoning and categorization. For reasoning, the issue is the relation between this proposal and one-process as well as two-process accounts of deduction and induction. For categorization, the issue is how the proposal would simultaneously explain both similarity-to-rule and rule-to-similarity shifts.

The article attempts to resolve the problem raised by richard sharvey's arguments supporting the thesis that temporal contexts present difficulties for quantification similar to those presented by alethic modal contexts. to this end a concept of temporally relativized identity for physical objects is introduced and explicated within a four-dimensional space-time view of the world. this in turn permits the introduction of the notion of "restricted" physical objects and the notion of "extended" physical objects. it is then shown how the use (...) of the introduced concepts together with careful attention to the details of temporal predication may permit the solution of the problem. (shrink)

Habermas's paradigm of communicative action is usually taken to be pretty much dominated by consensus, "Yes-saying." What if this were a radically one-sided perception? We take up this unorthodox position by arguing that "no-saying" in this paradigm is typically overlooked and underemphasized. To demonstrate this, we consider how negativity is figured at the most basic onto-ethical level in communicative action, as well as expressed in civil disobedience, a phenomenon to which Habermas assigns the remarkable role of "touchstone" (Prufstein) of constitutional (...) democracy. Once the importance of no-saying is drawn out, the paradigm looks distinctly less hostile to dissensus and agonism in democratic life. (shrink)

A functioning democracy depends on the free flow of information in the marketplace of ideas, creating an informed citizenry that can engage in public debate.This study examines the most-used online news portal, Yahoo!, to determine if the news media industry can be simultaneously profitable and socially responsible, providing the public with news that is both informative and engaging in an increasingly global world.

Faced with troubling professional decisions in his long and successful career as a psychiatrist, M. O'C. Drury turned for direction to the philosophical work of his teacher and friend, Ludwig Wittgenstein. Of particular concern to Drury were the situations in which psychiatrists were expected to differentiate between instances of madness that were religious in form and instances of genuine religious experience that, for their oddity, landed believers in psychiatric consulting rooms. In this essay we consider the special orientation Wittgenstein's philosophy (...) gave Drury, for example the way in which Drury came to understand how even his search for a principle of differentiation between madness and religion was misleading and contrary to his own practice—how it involved ‘sitting back in a cool hour and attempting to solve this problem as a pure piece of theory. To be the detached, wise, external critic’ and not see himself and his own manner of life ‘as intimately involved in the settlement of this question.’. (shrink)

Vaccine hesitancy is a major public health challenge, and racial disparities in the acceptance of vaccines is a particular concern. In this essay, we draw on interviews with mothers of Black male adolescents to offer insights into the reasons for the low rate of vaccination against the human papillomavirus among this group of adolescents. Based on these conversations, we argue that increasing the acceptance of HPV and other vaccines cannot be accomplished merely by providing people with more facts. Instead, we (...) must address the pervasive racial discrimination in the United States that undermines trust in social institutions, including the health care system. In the short term, it may be helpful to increase the number of clinicians of color working in the health system, but more fundamental changes are required. The U.S. must adopt and implement policies that dismantle structural racism if it hopes to produce greater trust and community‐oriented thinking on behalf of people who have been exploited for centuries. (shrink)

Recent advances in wireless data transmission technology have the potential to revolutionize clinical neuroscience. Today sensing-capable electrical stimulators, known as “bidirectional devices”, are used to acquire chronic brain activity from humans in natural environments. However, with wireless transmission come potential failures in data transmission, and not all available devices correctly account for missing data or provide precise timing for when data losses occur. Our inability to precisely reconstruct time-domain neural signals makes it difficult to apply subsequent neural signal processing techniques (...) and analyses. Here, our goal was to accurately reconstruct time-domain neural signals impacted by data loss during wireless transmission. Towards this end, we developed a method termed Periodic Estimation of Lost Packets. PELP leverages the highly periodic nature of stimulation artifacts to precisely determine when data losses occur. Using simulated stimulation waveforms added to human EEG data, we show that PELP is robust to a range of stimulation waveforms and noise characteristics. Then, we applied PELP to local field potential recordings collected using an implantable, bidirectional DBS platform operating at various telemetry bandwidths. By effectively accounting for the timing of missing data, PELP enables the analysis of neural time series data collected via wireless transmission—a prerequisite for better understanding the brain-behavior relationships underlying neurological and psychiatric disorders. (shrink)